e-book The Chemistry of Attraction

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Articles

  1. Sexual Chemistry: Why Our Bodies Understand Love Before Our Brains Do
  2. The Chemistry of Attraction - Catalyst
  3. Bear in mind the infatuation phase
  4. Taking Birth-Control Pills May Mask the Signals That Draw the Sexes Together, Research Shows

The chemistry is amplified even more when both laugh together, according to the study. When something is familiar, it becomes comfortable, and we are drawn to it. It sounds kind of creepy, but we tend to be attracted to people who look similar to us. According to research published in the journal Personality and Social Psychology Bulletin , we are attracted to people who resemble our parents or ourselves.

Chemistry behind Love

How much you reveal to a potential partner can set the tone for some crazy chemistry. Disclosing personal information can create a sense of intimacy , but revealing too much too soon can take away that feeling of excitement, according to Psychology Today.

Sexual Chemistry: Why Our Bodies Understand Love Before Our Brains Do

A t the beginning of a relationship, individuals tend to discuss a wide array of topics, and if theses conversations are rewarding, people begin to disclose more personal information, which can lead to chemistry. Length Of Eye Contact. Shared Sense Of Humor. Sense Of Familiarity. Physical Similarity. Chemokine receptors are percent homologous and are named according to the chemokine subfamily with which they interact. There are also miscellaneous receptors that don't yet fit into any group.

Fewer receptors than chemokines have been identified, but these receptors have distinct and overlapping expression.


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One receptor can bind multiple chemokines and be expressed on a variety of different cells. Additionally, each cell can react to various chemokines by expressing a single receptor. The potential benefit of this promiscuity is the recruitment of multiple cell types to sites of inflammation. The following sections will examine some of the commercially available assays and tools to analyze chemokine receptors. Several companies now sell purified chemokines for use in assorted assays.


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This process starts with a design stage based on the actual or predicted amino acid sequence of the protein. At this stage it is possible to incorporate protein modifications such as carbohydrates, fluorescent tags, polymers, fatty acids, cofactors, FRET dye pairs, affinity tags, and NMR or X-ray crystallography labels.

The Chemistry of Attraction - Catalyst

Once the design of the protein is established, peptide segments are made by total chemical synthesis. The segments are next linked using native chemical ligation to yield the full-length polypeptide chain. Lastly, the chain is refolded to create the functional protein product. Compared to biological production, which can take months, Gryphon's technology can produce functional proteins in days. This extensively validated technology generates high yields of ultrapure homogeneous proteins that are endotoxin free.

Another company that features purified chemokines and chemokine receptors is Chemicon International of Temecula, Calif. These purified chemokines and receptors can be used as standards for western blot analysis or as blocking peptides. Becton Dickinson PharMingen offers several recombinant chemokines from Escherichia coli or insect cell preps for use in functional assays.

BioSignal and Receptor Biology Inc. Membranes are typically packaged as frozen suspensions. Homogeneity between receptors is wonderful for cloning novel receptors but presents a serious problem for antibody detection--close family ties lead to cross-reactivity. Much to the relief of chemokine researchers, several companies market antibodies for chemokines and chemokine receptors.

Bear in mind the infatuation phase

And Assay We Go The following section will discuss several different types of assays that can be used to study chemokine receptors. As with any protocol, these general assays can be customized to suit the researcher. Once a chemokine receptor has been cloned, activity can be studied after stable transfection into a cell line. Some receptor and ion-channel activity assays involve taking cells in suspension or a monolayer of cells grown on a support and "loading" the cells with a fluorescent dye that binds calcium in the cells.

Once the cells are loaded with dye, the suspension is placed in a fluorescence spectrometer or plate reader that detects calcium mobilization upon exposure to the receptor's ligand. Chemotaxis, the movement of cells in response to a signal, is another method for examining the activity of chemokine receptors. Specialized media and culture supplements can be added to the coated inserts in an in vitro system to analyze leukocyte migration and adhesion in response to a chemokine. In addition to examining migration, the effects of anti-inflammatory agents can also be studied.

Neutralizing antibodies to chemokines or receptors can be used to prevent chemotaxis. Disposable and nondisposable chambers for chemotaxis are also available through Neuro Probe. A whole cell-binding assay can determine receptor density on the cell surface and the specific binding of a chemokine to its receptor. Cells are grown and resuspended to a particular cell density in growth medium. Labeled ligand in this case I-chemokine is added to the cells, and unlabeled chemokine is added in various concentrations as a competitor.

Chemokine receptor membrane preps and neutralizing chemokine antibodies can be used in competition assays. After incubation, the cells are washed, counts are read on a scintillation counter, and specific binding is determined.

Taking Birth-Control Pills May Mask the Signals That Draw the Sexes Together, Research Shows

This method also can be modified to use nonisotopic chemokines. The accompanying table lists companies that sell purified proteins and antibodies useful for studying chemokine receptor function. Be sure to check with these companies frequently as the rapid advancement of chemokine receptor research is spawning new reagents nearly everyday.